2 prep
FavorFilter Plasmid DNA Midi Kit - Endotoxin Removal (sample size: 10~30 ml culture cells; endotoxin removal buffer provided)

Principle: spin column (silica matrix)
Sample size: up to 15 ml cell culture
Operation time: < 60 minutes
Binding capacity: up to 200 µg/ column
Column applicability: centrifugation and vaccum

Materials and equipmentsupplied by user:
1. Pipettes and pipette tips
2. vortex
3. 100% ethanol
4. Swing bucket centrifuge for 15 ml centrifuge tube (4,500 ~ 6,000 x g) for centrifuge protocol
5. Vacuum manifold for vacuum protocol

Important Notes:
1. Store RNase A at -20 °C upon recipit of kit.
2. Add 0.5 ml of PDEA Buffer to the RNase A tube, vortex the tube to dissolve the RNase A well. Transfer the total RNase A mixture back to the PDEA bottle. Mix well by vortexing and store the PDEA buffer at 4 °C.
3. Buffer provided in this kit contain irritants. Wear gloves and lab coat when handling these buffers.
4. Check PDEB Buffer before use to see if any precipitate formed, If yes, warm Buffer PEDB in a 37 ºC waterbath to dissolve precipitates.
5. To avoid acidification of PDEB Buffer from CO2 in the air. Close the bottle immediately after use.
6. For centrifuge protocol, the centrifuge rotor for 15 ml tube should be swig-bucket type to make the plasmid bind symmetrically to the membranes.