24 prep
FavorPrep™ Blood/Cultured Cell Total RNA Maxi Kit (sample: 10 µl fresh whole blood, plasma, buffy coat; or cultured cells of animal, fungus or bacteria)

FavorPrep Blood/ Cultured Cell Total RNA Extraction Maxi Kit is desigened for extraction of total RNA from whole blood and cultured cells. Some specially modified protocols are developed for other samples, such as bacteria and yeast. This method first lyses cells by using a chaotropic salt , then binds RNA to silica-based membranes, washes RNA with ethanol-contained wash buffer and then elutes purified RNA by RNase-free Water. It takes 60 min for an entire procedure, and the purified RNA is ready for RT-PCR, northern blotting, primer extension and cDNA library contruction.

Important Notes:
1. Make sure everything is RNase-free when handling RNA.
2. Buffers provided in this system contain irritants. Wear gloves and lab coat when handling hese buffers.
3. Pipet a required volume of FARB Buffer to another RNase-free container and add 10 µl ß-mercaptoethanol (ß-ME) per 1ml FARB Buffer before use.
4. Add required volume of RNase-free ethanol (96~100%) to Wash Buffer 2 as bottle indicated when first open.
5. Dilute RNase-free DNase 1 in reaction buffer (150mM NaCl, 1 mM MgCl2, 10 mM Tris HCl, pH 7.5) to final conc. = 2KU/ml. (1 ml /preparation)
6. Use a centrifuge with a swinging bucket rotor for 15ml (Midi) or 50ml (Maxi) in all centrifugation steps. The maximum speed should be 3500-5000 rpm or 3000-5000 x g.