Name | : | FavorPrep™ Plant Total RNA Maxi Kit (sample size: up to1 g of plant tissue, fruit or seed) |
Cat NO | : | Cat NO: FAPRK 000-Maxi |
Sample Amount: 500 mg (up to 1 g) plant tissue or 5~10 X 10 plant
cells
Operation time: about 45~60 min
Binding Capacity: up to 1000 µg total RNA
Expected Yield: up to 50~300 µg total RNA from young leave
Elution volume: 500 µl
Important Notes:
1. Make sure everything is RNase-free when handling RNA.
2. Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers.
3. Pipet 5 ml of FARB Buffer to another RNase-free container and add 50 µl of ß-mercaptoethanol (ß-ME) before
every preparation.
4. Add required amount of ethanol (96-100%) Wash Buffer 2 when first open.
5. Dilute RNase-free DNase 1 in reaction buffer (1M NaCl, 10mM MnCl2, 20mM Tris-HCl, pH7.0 at 25 °C) to final
conc.= 0.5U/µl.
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PingTung Agricultural Biotechnology Park
No.37,Nong-Ke Rd., Ping-Tung 908,
Taiwan
Tel: +886-8-762-1829
Fax: +886-8-762-0791
Email: order@favorgen.com
Shuttleworthstraße 19, A-1210 Vienna
phone: +43 (0)1 292 82 80
fax: +43 (0)1 292 82 80-88
Email: r.englisch@favorgen-europe.com